Immobilized Fluorescent Probes for Simultaneous Multiple Protease Detection
نویسندگان
چکیده
A new concept for simultaneous detection of two model proteases based on immobilized fluorescently labelled peptides was developed and evaluated. Each probe composed a carrier modified by poly(ethylene glycol) (PEG) chains, specifically cleavable linker, fluorescent dye incorporated in the peptide tail. Based single excitation–double emission fluorescence response liberated fluorophores caused enzymatic cleavage, presence or both mixture unambiguously determined an experimentally established concentration range. Among tested solid supports, Rink Amide PEGA resin recognized as most suitable choice from perspective on-resin enzyme assays.
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ژورنال
عنوان ژورنال: Chemosensors
سال: 2021
ISSN: ['2227-9040']
DOI: https://doi.org/10.3390/chemosensors9060119